| National Science Teachers AssociationEIN 52-6055229 | Education | VA | $26,368,291Large | 80.44 |
| National Sleep FoundationEIN 41-1678336 | Health | VA | $4,114,158Mid-sized | 89.94 |
| National Sporting Library & MuseumEIN 54-6053662 | Arts, Culture, Humanities | VA | $2,059,740Small | 82.28 |
| National Wildlife FederationEIN 53-0204616 | Animals | VA | $73,731,937Large | 81.19 |
| NatureServeEIN 52-1884438 | Environment | VA | $8,421,554Mid-sized | 84.93 |
| Navy SEAL FoundationEIN 31-1728910 | Human Services | VA | $15,895,708Large | 100.00 |
| Navy-Marine Corps Relief SocietyEIN 53-0204618 | Human Services | VA | $27,989,419Large | 89.39 |
| Negative Population GrowthEIN 46-4868482 | Research and Public Policy | VA | $816,014Small | 78.66 |
| NetHopeEIN 20-1782011 | International | VA | $9,628,983Mid-sized | 87.58 |
| Norfolk Botanical GardenEIN 54-0788933 | Environment | VA | $5,367,710Mid-sized | 85.06 |
| NumbersUSA Education and Research Foundation EIN 47-0865426 | Human and Civil Rights | VA | $6,623,333Mid-sized | 98.23 |
| Old Dominion Athletic FoundationEIN 54-6051933 | Education | VA | $7,930,953Mid-sized | 89.39 |
| Operation Blessing InternationalEIN 54-1382657 | International | VA | $272,605,727Large | 82.43 |
| Operation First ResponseEIN 20-1622436 | Human Services | VA | $803,443Small | 91.16 |
| Organization for Autism ResearchEIN 54-2062167 | Health | VA | $1,326,919Small | 95.05 |
| Orphan HelpersEIN 54-1995429 | Religion | VA | $605,140Small | 80.16 |
| ORPHANetworkEIN 54-1983817 | International | VA | $5,817,562Mid-sized | 95.47 |
| Our Military KidsEIN 56-2483648 | Human Services | VA | $2,091,157Small | 93.65 |
| Patient Advocate FoundationEIN 54-1806317 | Health | VA | $186,165,629Large | 96.66 |
| Patient Services IncorporatedEIN 54-1596178 | Health | VA | $110,091,811Large | 92.22 |
| Patrick Henry Family ServicesEIN 54-0660819 | Human Services | VA | $5,477,543Mid-sized | 81.00 |
| Peninsula Metropolitan Young Men's Christian AssociationEIN 54-0524905 | Human Services | VA | $18,463,775Large | 91.42 |
| People for the Ethical Treatment of AnimalsEIN 52-1218336 | Animals | VA | $64,212,553Large | 80.80 |
| Physicians for PeaceEIN 54-1532165 | International | VA | $1,059,930Small | 82.56 |
| Population Research InstituteEIN 54-1819935 | Research and Public Policy | VA | $1,368,353Small | 62.84 |
| Positive Alternative Radio, Inc.EIN 52-1440112 | Religion | VA | $6,423,088Mid-sized | 77.56 |
| Preservation VirginiaEIN 54-0568800 | Arts, Culture, Humanities | VA | $3,746,830Mid-sized | 86.84 |
| Prevent Cancer FoundationEIN 52-1429544 | Health | VA | $7,131,197Mid-sized | 91.24 |
| Prison Fellowship MinistriesEIN 62-0988294 | Religion | VA | $37,459,967Large | 75.58 |
| Project HOPEEIN 53-0242962 | International | VA | $99,239,752Large | 83.84 |
| Purple Heart FoundationEIN 59-3184919 | Human Services | VA | $32,780,857Large | 28.59 |
| Rainforest TrustEIN 13-3500609 | Environment | VA | $16,200,766Large | 94.69 |
| Rappahannock United WayEIN 54-6042936 | Community Development | VA | $3,672,925Mid-sized | 88.93 |
| RareEIN 23-7380563 | Environment | VA | $25,106,435Large | 92.29 |
| Reaching Unreached NationsEIN 54-1563242 | Religion | VA | $6,077,734Mid-sized | 64.50 |
| Rescue Mission of RoanokeEIN 54-0573900 | Human Services | VA | $4,755,474Mid-sized | 90.54 |
| Research!AmericaEIN 52-1609875 | Research and Public Policy | VA | $3,687,258Mid-sized | 80.61 |
| Richmond Ballet, The State Ballet of VirginiaEIN 54-6049848 | Arts, Culture, Humanities | VA | $7,064,503Mid-sized | 90.94 |
| Richmond Friends of the HomelessEIN 54-1601100 | Human Services | VA | $1,759,090Small | 78.63 |
| Richmond HillEIN 54-1360535 | Religion | VA | $1,404,738Small | 77.94 |
| Richmond Performing Arts AllianceEIN 54-2028439 | Arts, Culture, Humanities | VA | $2,309,500Small | 89.80 |
| Richmond SPCAEIN 54-0506328 | Animals | VA | $6,971,781Mid-sized | 91.87 |
| Richmond SymphonyEIN 54-6024033 | Arts, Culture, Humanities | VA | $5,127,052Mid-sized | 78.97 |
| Roanoke Symphony OrchestraEIN 54-6019736 | Arts, Culture, Humanities | VA | $1,791,144Small | 78.19 |
| Roanoke Valley SPCAEIN 54-0679796 | Animals | VA | $1,054,949Small | 89.30 |
| Robert E. Lee Memorial AssociationEIN 54-0536105 | Arts, Culture, Humanities | VA | $3,723,205Mid-sized | 85.47 |
| Science Museum of Western VirginiaEIN 54-1023953 | Arts, Culture, Humanities | VA | $871,912Small | 88.97 |
| Second StoryEIN 54-0899463 | Human Services | VA | $3,085,749Small | 91.02 |
| SHRM FoundationEIN 34-6610067 | Education | VA | $2,302,725Small | 86.65 |
| Southern Environmental Law CenterEIN 52-1436778 | Environment | VA | $26,551,471Large | 100.00 |
| SPARKEIN 54-1498824 | Education | VA | $4,425,085Mid-sized | 78.96 |
| Special Olympics VirginiaEIN 54-1013637 | Human Services | VA | $4,833,080Mid-sized | 89.91 |
| Spina Bifida AssociationEIN 58-1342181 | Health | VA | $1,821,008Small | 82.11 |
| Spirit of AmericaEIN 20-1687786 | International | VA | $3,626,101Mid-sized | 96.83 |
| Sports Outreach InstituteEIN 54-1479310 | Religion | VA | $1,923,423Small | 85.93 |
| State Policy NetworkEIN 57-0952531 | Research and Public Policy | VA | $12,797,260Mid-sized | 88.07 |
| Student Conservation Association EIN 91-0880684 | Environment | VA | $43,289,688Large | 89.65 |
| Tahirih Justice CenterEIN 54-1858176 | Human and Civil Rights | VA | $7,001,173Mid-sized | 88.40 |
| Taubman Museum of ArtEIN 54-6026841 | Arts, Culture, Humanities | VA | $4,213,597Mid-sized | 88.16 |
| TechnoServeEIN 13-2626135 | International | VA | $85,806,073Large | 86.73 |